Overview & Features
S Plan Fluor LWD – Plastic-compatible high-resolution, large-FOV objectives
Features
CFI S Plan Fluor LWD 20XC and CFI S Plan Fluor LWD ADM 20XC objectives for biological microscopes are designed for stem cell applications and drug design. With long working distances*1 and high numerical apertures, these objectives provide bright, high-resolution images through plastic cell-culture dishes.
The CFI S Plan Fluor LWD 20XC is suitable for brightfield, fluorescence and DIC observation, and the CFI S Plan Fluor LWD ADM 20XC can be used for brightfield, fluorescence and phase contrast observation.
*1 Working distance: The distance from the tip of the objective lens to the observation target when the target is in focus. A long working distance has numerous advantages, such as prevention of contact with the sample when changing objectives.
Long working distances and high NA
The new objectives provide long working distances of 1.3 mm to 2.3 mm, which are compatible with observations using thick-bottomed plastic dishes and observations of thick observation targets. In addition, the high NA of 0.70 enables rapid acquisition of sharp images even during fluorescence observation, which requires bright images.
25 mm FOV supports wide visual field observation
The new objectives provide 20X observations with an FOV of 25 mm*2 when used together with the ECLIPSE Ti2 Inverted Research Microscope. They allow effective observation of wide areas and rapid acquisition of large amounts of observation data, enabling high cell screening and image stitching*3 throughput.
*2 FOV (field of view: the diameter of the observable image in a microscope)
*3 Image stitching: an image processing method that combines neighboring images to create one large
Specifications
| Model | Dimensions | Transmittance | NA | W.D. (mm) |
Cover glass thickness | Correction ring | Observation |
|---|---|---|---|---|---|---|---|
| CFI S Plan Fluor LWD 20XC | Diagram | graph | 0.70 | 1.3-2.3 | 0-1.8 | ∨ | BF, DF (Dry/Oil), DIC, POL, FL (visible light, UV) |
| CFI S Plan Fluor LWD ADM 20XC | Diagram | graph | 0.70 | 1.3-2.3 | 0-1.8 | ∨ | BF, DF (Dry/Oil), PH, FL (visible light, UV) |
BF: Brightfield
DF: Darkfield
PH: Phase contrast
POL: Simple polarizing
FL: Fluorescence
*Possible but not recommended
**External phase contrast observation is possible with Eclipse Ti2-E
